Association between adeno‐associated virus genomic titers and intracellular plasmid levels

The recombinant adeno-associated viral (rAAV) vector is one of the most effective vectors in gene therapy because its low immunogenicity, high transduction efficiency, broad tissue specificity, and long-term transgene expression ability. HEK293T cells are widely used to produce rAAV vectors, acquisition rAAV-producing essential processes for higher production. To acquire such cells, a method identify with protein was developed; however, relationship between increased production has not been sufficiently studied date. We fluorescent ZsGreen1 as transgene, subdivided adherent according intensity protein, reconfirmed amount intracellular plasmid. found that produced correlated but may be also might necessarily large amounts vector. Based on these results, it suggested plasmid copy number could new marker producing levels